DNA ligases seal five′-PO4 and three′-OH polynucleotide ends via three nucleotidyl transfer ways involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase dysfunction underlies human genetic ailment syndromes. Crystal structures of DNA ligases sure to nucleotide and nucleic acid substrates have illuminated how ligase response chemistry is catalyzed, how ligases identify harmed DNA ends, And just how protein area movements and active-site transforming are used to choreograph the top-joining pathway.
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